Chromaffin Cells: Methods and Protocols by Ricardo Borges (English) Hardcover Bo

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Chromaffin Cells

by Ricardo Borges

This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

FORMAT Hardcover LANGUAGE English CONDITION Brand New

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This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Table of Contents

 Part I   Culturing and morphology 1. Rat pheochromocytoma PC12 cells in cultureChin-Tien Wang 2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantationAlejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo 3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cellsKeita Harada, Hidetada Matsuoka, Masumi Inoue 4. Transmission electron microscopy: a method for studying the adrenochromaffin cellsAnna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia 5. Immunogold for protein location in chromaffin cellsRafael Luján, Rocio Alfaro-Ruiz and Carolina Aguado 6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopyChung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu Part II   Stimulus-secretion coupling 7. Online detection of catecholamine release from the perfused rat adrenal gland Ricardo Borges, Ana de la Iglesia and Antonio G. García 8. On-line recording of perifused chromaffin cellsRicardo De Pascual and Luis Gandía 9. Recording of chromaffin cell electrical activity in situ in acute adrenal slicesNathali Guerineau 10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive RatsTzitzitlini Alejandre-García, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Millán-Aldaco and Arturo Hernández-Cruz. 11. Measurements of calcium in chromaffin cell organelles using targeted aequorins.Jaime Santo-Domingo, Pilar Álvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier Álvarez 12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurementsMuhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg 13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cellElizabeth P. Seward and Robert C. Wykes 14. Quantal release analysis of electrochemically active molecules using single-cell amperometry Natalia Domínguez, Pablo Montenegro and J. David Machado 15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAsGiulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli 16. Vesicle collision protocols for the study of quantum size and exocytotic fraction releasedSoodabeh Majdi, Alex S. Lima and Andrew Ewing 17. Patch Amperometry and Intracellular Patch ElectrochemistryEugene V. Mosharov and Manfred Lindau 18. Artificial cells for dissecting exocytosisYuanmo Wang and Ann-Sofie Cans Part III   Chromaffin granules and membrane traffic 19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cellsArlek Gonzalez-Jamett, María Constanza Maldifassi, Ana María Cárdenas 20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells José Villanueva, Yolanda Gimenez-Molina and Luis M. Gutiérrez 21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
Adekunle T. Bademosi and Frédéric A. Meunier 
 Part IV   Miscellanea 22. Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein Kechun Tang and Sushil K. Mahata 23. Quantification of chromogranin A and its fragments in biological fluids Flavio Curnis, Barbara Colombo and Angelo Corti 24. Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds María F. Cano Abad and Manuela G. López

Feature

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Details ISBN1071626701 Short Title Chromaffin Cells Series Methods in Molecular Biology Language English Year 2022 ISBN-10 1071626701 ISBN-13 9781071626702 Format Hardcover Subtitle Methods and Protocols Series Number 2565 Publisher Springer-Verlag New York Inc. Edition 1st Imprint Springer-Verlag New York Inc. Place of Publication New York, NY Country of Publication United States Pages 373 Author Ricardo Borges Publication Date 2022-10-08 AU Release Date 2022-10-08 NZ Release Date 2022-10-08 US Release Date 2022-10-08 UK Release Date 2022-10-08 Edited by Ricardo Borges Illustrations 68 Illustrations, color; 21 Illustrations, black and white; XIV, 373 p. 89 illus., 68 illus. in color. Edition Description 1st ed. 2023 Alternative 9781071626733 DEWEY 573.4 Audience Professional & Vocational

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TheNile_Item_ID:141668141;
  • Condition: Brand New
  • Format: Hardcover
  • Language: English
  • ISBN-13: 9781071626702
  • Author: Ricardo Borges
  • Book Title: Chromaffin Cells

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