This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
FORMAT Hardcover LANGUAGE English CONDITION Brand NewThis volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Part I Culturing and morphology 1. Rat pheochromocytoma PC12 cells in cultureChin-Tien Wang 2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantationAlejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo 3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cellsKeita Harada, Hidetada Matsuoka, Masumi Inoue 4. Transmission electron microscopy: a method for studying the adrenochromaffin cellsAnna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia 5. Immunogold for protein location in chromaffin cellsRafael Luján, Rocio Alfaro-Ruiz and Carolina Aguado 6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopyChung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu Part II Stimulus-secretion coupling 7. Online detection of catecholamine release from the perfused rat adrenal gland Ricardo Borges, Ana de la Iglesia and Antonio G. García 8. On-line recording of perifused chromaffin cellsRicardo De Pascual and Luis Gandía 9. Recording of chromaffin cell electrical activity in situ in acute adrenal slicesNathali Guerineau 10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive RatsTzitzitlini Alejandre-García, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Millán-Aldaco and Arturo Hernández-Cruz. 11. Measurements of calcium in chromaffin cell organelles using targeted aequorins.Jaime Santo-Domingo, Pilar Álvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier Álvarez 12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurementsMuhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg 13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cellElizabeth P. Seward and Robert C. Wykes 14. Quantal release analysis of electrochemically active molecules using single-cell amperometry Natalia Domínguez, Pablo Montenegro and J. David Machado 15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAsGiulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli 16. Vesicle collision protocols for the study of quantum size and exocytotic fraction releasedSoodabeh Majdi, Alex S. Lima and Andrew Ewing 17. Patch Amperometry and Intracellular Patch ElectrochemistryEugene V. Mosharov and Manfred Lindau 18. Artificial cells for dissecting exocytosisYuanmo Wang and Ann-Sofie Cans Part III Chromaffin granules and membrane traffic 19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cellsArlek Gonzalez-Jamett, María Constanza Maldifassi, Ana María Cárdenas 20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells José Villanueva, Yolanda Gimenez-Molina and Luis M. Gutiérrez 21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
Adekunle T. Bademosi and Frédéric A. Meunier
Part IV Miscellanea 22. Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein Kechun Tang and Sushil K. Mahata 23. Quantification of chromogranin A and its fragments in biological fluids Flavio Curnis, Barbara Colombo and Angelo Corti 24. Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds María F. Cano Abad and Manuela G. López
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
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